HIC Chromatography Media Nano Phenyl-15L
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- L/C, T/T
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Contact Now| Payment Type: | L/C,T/T |
|---|---|
| Incoterm: | FOB,CFR,CIF |
| Transportation: | Ocean,Air |
Nanomicro Tech offers a series of hydrophobic interaction chromatography (HIC) media for high efficiency separation and purification of proteins and peptides.The salt concentration needed depends on the protein hydrophobicity and solubility. HIC is particularly attractive for protein purification after ion exchange and affinity chromatography, where the sample with high salt concentration can often be applied directly to HIC. By combining HIC with ion exchange or affinity chromatography, the purification of biomolecules can be completed efficiently.
HIC is used to separate proteins on the basis of relative hydrophobicity. The unique feature of HIC is that proteins bind at high salt concentration and elute at low salt concentration. While in reversed phase column chromatography (RPC), desorption is often promoted by the addition of an increasing amount of organic solvent. Therefore gentle binding and elution condition of HIC allows a better retaining of the biologic activity of the target protein than RPC in which organic solvents are applied. The strength of the hydrophobic interaction is influenced strongly by the nature of the salt components in the mobile phase.
Features and Benefits
|
Features |
Benefits |
|
Highly uniform particle size |
Easy column packing, high resolution |
|
Optimised pore structure and surface bonding |
High loading capacity and high selectivity |
|
Superior chemical and pH stability |
Easy cleaning, long lifetime |
|
No filter impurities |
Eliminate non-specific bindings |
|
High mechanical strength |
Low back pressure, High linear velocity |
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Large-scale manufacturing capability |
Consistent product quality and supply |
|
Neutral hydrophilic modification |
high yield |
Highly uniform particle size
Uni®HIC are designed to deliver high resolution and high recovery for both lab and large-scale purification. Highly uniform particle sizes of 15, 30, or 80 μm with a narrow particle size distribution are available.
Superior chemical and pH stability
|
NanoHR Phenyl-15L § Uni®HR Butyl-30L Phenyl-15L |
Comparison of column efficiency after treatment with acid and base
High Binding Capacity
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Products List
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|
Series |
Product Name |
Diameter |
Pore Szie |
Functional Group |
Features/ Benefits |
Application |
|
μm |
Å |
|||||
|
UniÒHR |
UniÒHR Phenyl-80L |
80 |
1000 |
Phenyl |
Eliminate non-specific bindings |
Ideal for high resolution seperation of proteins and peptides |
|
UniÒHR Phenyl-30L |
32 |
1000 |
Phenyl |
|||
|
UniÒHR Phenyl-60S |
60 |
500 |
Phenyl |
|||
|
UniÒHR Phenyl-30S |
35 |
500 |
Phenyl |
|||
|
UniÒHR Butyl-80L |
80 |
1000 |
Butyl |
|||
|
UniÒHR Butyl-30L |
32 |
1000 |
Butyl |
|||
|
UniÒHR Butyl-60S |
60 |
500 |
Butyl |
|||
|
UniÒHR Butyl-30S |
35 |
500 |
Butyl |
|||
|
NanoHR |
NanoHR Phenyl-15L |
15 |
1000 |
Phenyl |
High resolution/ High mechanical strength /High chemical stability |
Ideal for fast and high polishing and analysis of proteins and peptides |
|
NanoHR Butyl-15L |
15 |
1000 |
Butyl |
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